Bio-Rad CFX96 Real-Time PCR System

Description

Use the CFX96 Deep Well Real-Time PCR Detection System for precise, reproducible real-time PCR. The system is composed of the CFX96 Deep Well Optical Reaction Module and the C1000 Touch Thermal Cycler Chassis. The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.

• Deep wells for large volumes
• Detection of up to 5 targets per well, plus a channel dedicated to singleplex FRET
• Protocol auto writer that generates an optimal protocol for your reaction components
• The thermal gradient that identifies optimal annealing temperature in a single run

The CFX96 Deep Well Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).

The optical system, with six filtered LEDs and six filtered photodiodes, collects data from all wells, detecting up to five targets per well. For single-color FAM and SYBR^® Green I, the fast scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

The large touch-screen interface has intuitive graphical programming and displays data traces in real-time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol auto writer quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.