Beckman Coulter FC500 Cytometer

Description

The TQ-Prep Workstation and IMMUNOPRE PReagent System together provide rapid, no-wash whole-blood sample preparation. Automation provides inter-sample and inter-laboratory consistency and reproducibility. A standard 32-tube carousel allows walk-away processing and perfectly complements both the XL-MCL and FC500 flow cytometers automatic sample loaders for the fastest sample throughput in the industry. Intended primarily for laboratories with a high test volume (at least 300 tests per month), the TQ-Prep uses the revolutionary patented lyse and fix technology introduced more than a decade ago with the Q-PREP Workstation and IMMUNOPREP Reagent System.

Lasers
Air-cooled Argon ion laser, 488nmRed Solid state laser, 631nm

Detectors

Forward Scatter and Side scatter detectors are photodiodes

Five photomultipliers collect the fluorescence signals at:

FL1 = 525 nm/40 (FITC, Alexa Fluor488, GFP, CFSE, Calcein, YFP, R123, Mito Green, Syto 14-18, YoPro3, Fluo4)

FL2 = 575 nm/40 (PE)

FL3 = 620 nm/20 (PE-TexasRed, Propidium Iodide PE-Alexa Flour 610)

FL4 = 675 nm/40 (Blue laser: Per-CP, Drad5 (DNA), Fura Red, 7-AAD, PE-Cy5; Red laser: APC, Cy5, Alexa Fluor 633, 647, 660 and 680, Drad5, Mito deep Red, ToPro3, ToTo3)

FL5 = 755 nm/40 (Blue laser: PE-Cy7, PE-Alexa Fluor 700; Red laser: APC-Cy7, APC-Alexa Fluor750, APC-H7)

Note: The blue laser and red laser of FC500 Flow Cytometer overlap each other. The combination of dye selection at FL4 and FL5 detectors can either be excited by blue laser or red laser